Hotspot

trnV-GAC-rrn16

Genome · 99345-99743 · 399 bp

Back to Crepidomanes

Pi 0.193

MarkerSeek score 84.00

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 399
variable_sites: 76
pi: 0.193
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	GGTGCAAGTCATCAGTTCGA	CCACTAGAAACACCACGAGG	50.0	55.0	58.2	57.9	531-1404 bp	1.0	84.4
trnV-GAC-rrn16_p2	GGTGCAAGTCATCAGTTCGA	GAGCCAGGATCAAACTCTCC	50.0	55.0	58.2	57.7	460-1333 bp	1.0	83.0
trnV-GAC-rrn16_p3	GGTGCAAGTCATCAGTTCGA	AGCCAGGATCAAACTCTCCA	50.0	50.0	58.2	58.3	459-1332 bp	1.0	82.9
trnV-GAC-rrn16_p4	AACTCAGCGGTAGAGTGTCA	CCACTAGAAACACCACGAGG	50.0	55.0	58.4	57.9	560-1433 bp	1.0	82.8
trnV-GAC-rrn16_p5	TGGTGCAAGTCATCAGTTCG	CCACTAGAAACACCACGAGG	50.0	55.0	58.5	57.9	532-1405 bp	1.0	82.1