Hotspot

rps8-rpl14

Genome · 86203-86691 · 489 bp

Back to Crepidomanes

Pi 0.245

MarkerSeek score 75.00

Primer available yes

Label rps8-rpl14

Feature Metadata

label_name: rps8-rpl14
feature_type: igs
region: Genome
length_bp: 489
variable_sites: 81
pi: 0.245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.67
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8-rpl14_p1	TGTTGGCATTTCGTAGAAGAGT	TAGCTCCTGAGGTATTGTAA	40.9	40.0	58.0	52.4	412-561 bp	1.0	52.0
rps8-rpl14_p2	TGTTGGCATTTCGTAGAAGAG	TAGCTCCTGAGGTATTGTAA	42.9	40.0	56.3	52.4	412-561 bp	1.0	52.0
rps8-rpl14_p3	GTTGGCATTTCGTAGAAGAGT	TAGCTCCTGAGGTATTGTAA	42.9	40.0	56.3	52.4	411-560 bp	1.0	52.0
rps8-rpl14_p4	TGTTGGCATTTCGTAGAAGA	TAGCTCCTGAGGTATTGTAA	40.0	40.0	55.0	52.4	412-561 bp	1.0	52.0
rps8-rpl14_p5	TGTTGGCATTTCGTAGAAGAGT	AGCTCCTGAGGTATTGTAA	40.9	42.1	58.0	52.3	411-560 bp	1.0	52.0