Hotspot

ndhC-trnV-UAC

Genome · 58532-59109 · 578 bp

Back to Crepidomanes

Pi 0.231

MarkerSeek score 83.40

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: Genome
length_bp: 578
variable_sites: 124
pi: 0.231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GAGGGCTAGATCGTGGTACT	CGCCGATGTCTCTCAAAAGA	55.0	50.0	58.0	58.0	1807-1883 bp	1.0	89.7
ndhC-trnV-UAC_p2	AAAGACGGGGCCATTTTCAT	CGCCGATGTCTCTCAAAAGA	45.0	50.0	58.1	58.0	1865-1941 bp	1.0	89.2
ndhC-trnV-UAC_p3	TCATATTGATGGTGCCAGCC	CGCCGATGTCTCTCAAAAGA	50.0	50.0	58.0	58.0	1849 bp	0.5	59.9
ndhC-trnV-UAC_p4	GATGGTGCCAGCCGTTATTA	CGCCGATGTCTCTCAAAAGA	50.0	50.0	58.0	58.0	1842 bp	0.5	59.7
ndhC-trnV-UAC_p5	TCTTACTAAAGACGGGGCCA	CGCCGATGTCTCTCAAAAGA	50.0	50.0	58.1	58.0	1872 bp	0.5	59.5