Hotspot

rps4-trnL-UAA

Genome · 54776-55580 · 805 bp

Back to Crepidomanes

Pi 0.189

MarkerSeek score 84.20

Primer available yes

Label rps4-trnL-UAA

Feature Metadata

label_name: rps4-trnL-UAA
feature_type: igs
region: Genome
length_bp: 805
variable_sites: 140
pi: 0.189
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnL-UAA_p1	GGTCCTCTATAACGAGACAT	GCGTCTACCATTTCGCCATA	45.0	50.0	53.4	58.1	850-856 bp	1.0	54.0
rps4-trnL-UAA_p2	GGTCCTCTATAACGAGACAT	TACCATTTCGCCATATCCCC	45.0	50.0	53.4	57.1	845-851 bp	1.0	52.0
rps4-trnL-UAA_p3	GGTCCTCTATAACGAGACAT	CTACCATTTCGCCATATCCCC	45.0	52.4	53.4	58.3	846-852 bp	1.0	52.0
rps4-trnL-UAA_p4	GGTCCTCTATAACGAGACAT	GCGTCTACCATTTCGCCAT	45.0	52.6	53.4	58.3	850-856 bp	1.0	52.0
rps4-trnL-UAA_p5	GGTCCTCTATAACGAGACAT	GCGTCTACCATTTCGCCATAT	45.0	47.6	53.4	58.6	850-856 bp	1.0	52.0