Hotspot

psbD-trnT-GGU

Genome · 44108-45260 · 1153 bp

Back to Crepidomanes

Pi 0.173

MarkerSeek score 85.90

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1153
variable_sites: 190
pi: 0.173
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	AGACTTTCCAATGGCAATGGT	GGCGTAAGTCATCGGTTCAA	42.9	50.0	58.1	58.3	1212-1357 bp	1.0	80.4
psbD-trnT-GGU_p2	GACTTTCCAATGGCAATGGTC	GGCGTAAGTCATCGGTTCAA	47.6	50.0	57.8	58.3	1211-1356 bp	1.0	79.3
psbD-trnT-GGU_p3	ACTTTCCAATGGCAATGGTCA	GGCGTAAGTCATCGGTTCAA	42.9	50.0	58.4	58.3	1210-1355 bp	1.0	78.1
psbD-trnT-GGU_p4	CTTTCCAATGGCAATGGTCA	GGCGTAAGTCATCGGTTCAA	45.0	50.0	56.6	58.3	1209-1354 bp	1.0	77.8
psbD-trnT-GGU_p5	ACTTTCCAATGGCAATGGTC	GGCGTAAGTCATCGGTTCAA	45.0	50.0	56.6	58.3	1210-1355 bp	1.0	77.8