Hotspot

trnE-UUC-trnG-GCC

Genome · 39347-39981 · 635 bp

Back to Crepidomanes

Pi 0.248

MarkerSeek score 85.90

Primer available yes

Label trnE-UUC-trnG-GCC

Feature Metadata

label_name: trnE-UUC-trnG-GCC
feature_type: igs
region: Genome
length_bp: 635
variable_sites: 130
pi: 0.248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 85.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnG-GCC_p1	GTCGGCTCCTTGAAAGAGAG	TCTTTGCCAAGGAGAAGACG	55.0	50.0	58.0	57.8	626-727 bp	1.0	90.6
trnE-UUC-trnG-GCC_p2	TCGGCTCCTTGAAAGAGAGA	TCTTTGCCAAGGAGAAGACG	50.0	50.0	58.1	57.8	625-726 bp	1.0	90.0
trnE-UUC-trnG-GCC_p3	ATGTCCTAGGCCTCTAGACG	TCTTTGCCAAGGAGAAGACG	55.0	50.0	57.7	57.8	606-707 bp	1.0	88.4
trnE-UUC-trnG-GCC_p4	CCTAGGCCTCTAGACGATGG	TCTTTGCCAAGGAGAAGACG	60.0	50.0	58.5	57.8	602-703 bp	1.0	87.0
trnE-UUC-trnG-GCC_p5	CTAGGCCTCTAGACGATGGG	TCTTTGCCAAGGAGAAGACG	60.0	50.0	58.5	57.8	601-702 bp	1.0	87.0