Hotspot

matK-rps16

Genome · 9224-13049 · 3826 bp

Back to Crepidomanes

Pi 0.2

MarkerSeek score 55.40

Primer available yes

Label matK-rps16

Feature Metadata

label_name: matK-rps16
feature_type: igs
region: Genome
length_bp: 3826
variable_sites: 119
pi: 0.2
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.16
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-rps16_p1	GAGTGCCAAATGAGCTCTCA	TTGTCTAGCCACAAGATCGC	50.0	50.0	57.9	58.0	767 bp	0.5	59.1
matK-rps16_p2	AGCTCTCAACCCAGATGGAT	TTGTCTAGCCACAAGATCGC	50.0	50.0	58.1	58.0	755 bp	0.5	59.0
matK-rps16_p3	GAGTGCCAAATGAGCTCTCA	GCATCGTCCAAAGAGCCTAA	50.0	50.0	57.9	58.0	1115 bp	0.5	59.0
matK-rps16_p4	AGCTCTCAACCCAGATGGAT	GCATCGTCCAAAGAGCCTAA	50.0	50.0	58.1	58.0	1103 bp	0.5	58.9
matK-rps16_p5	GAGTGCCAAATGAGCTCTCA	GTGGAATAAGGCTTACGGCA	50.0	50.0	57.9	58.0	922 bp	0.5	58.9