Hotspot

ycf1-1

SSC · 154401-158884 · 4484 bp

Back to Crepidium

Pi 0.00918

MarkerSeek score 46.40

Primer available yes

Label ycf1-1

Feature Metadata

label_name: ycf1-1
feature_type: gene
region: SSC
length_bp: 4484
variable_sites: 41
pi: 0.00918
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-1_p1	CTCTCTGCCCATCCAATTCC	CGCAGAATTTCACCGTCAAC	55.0	50.0	58.0	58.0	574-580 bp	1.0	80.5
ycf1-1_p2	CTCTCTGCCCATCCAATTCC	TCAACGGACCATATCGAGGA	55.0	50.0	58.0	57.9	704-710 bp	1.0	80.4
ycf1-1_p3	CTCTCTGCCCATCCAATTCC	ATTCCGCAGAATTTCACCGT	55.0	45.0	58.0	58.2	578-584 bp	1.0	79.6
ycf1-1_p4	CTCTCTGCCCATCCAATTCC	CCGCCCAGTTTATCGACTTT	55.0	50.0	58.0	58.3	972-978 bp	1.0	78.9
ycf1-1_p5	AGAACCAAAGGGAAGTTCCG	CGCAGAATTTCACCGTCAAC	50.0	50.0	57.7	58.0	1084-1090 bp	1.0	78.3