Hotspot

ndhF-rpl32

SSC · 142763-143668 · 906 bp

Back to Crepidium

Pi 0.0188

MarkerSeek score 69.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 906
variable_sites: 17
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGCGGAAAAAGGGGTAT	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	57.9	57.3	1048-1105 bp	1.0	50.3
ndhF-rpl32_p2	GGAAGCGGAAAAAGGGGTAT	AGAAAAAGCTTTTACTGCCTCT	50.0	36.4	57.9	56.2	1055-1112 bp	1.0	49.7
ndhF-rpl32_p3	GGAAGCGGAAAAAGGGGTAT	AAGAAAAAGCTTTTACTGCCTCT	50.0	34.8	57.9	56.9	1056-1113 bp	1.0	47.3
ndhF-rpl32_p4	AACTGGAAGCGGAAAAAGGG	GCTTTTACTGCCTCTAAAGATCC	50.0	43.5	58.7	57.3	1052-1109 bp	1.0	46.0
ndhF-rpl32_p5	GGAAGCGGAAAAAGGGGTAT	GAAAAAGCTTTTACTGCCTCT	50.0	38.1	57.9	54.7	1054-1111 bp	1.0	45.6