Hotspot

petA-psbJ

LSC · 91351-92363 · 1013 bp

Back to Crepidium

Pi 0.0131

MarkerSeek score 66.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1013
variable_sites: 13
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTGATTTTGGCAC	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.0	57.3	1125-1167 bp	1.0	76.4
petA-psbJ_p2	GGCATCTGTGATTTTGGCAC	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.0	57.3	1126-1168 bp	1.0	76.3
petA-psbJ_p3	ACGAGTCCAAGGCCTTCTAT	TATTCCGGGTTGGGTTCATC	50.0	50.0	58.1	57.3	1152-1194 bp	1.0	75.3
petA-psbJ_p4	ACGAGTCCAAGGCCTTCTAT	CTATTCCGGGTTGGGTTCAT	50.0	50.0	58.1	57.3	1153-1195 bp	1.0	75.2
petA-psbJ_p5	GGCATCTGTGATTTTGGCAC	ATTCCGGGTTGGGTTCATCT	50.0	50.0	58.0	59.0	1124-1166 bp	1.0	74.2