Hotspot

trnF-GAA-ndhJ

LSC · 76271-76886 · 616 bp

Back to Crepidium

Pi 0.012

MarkerSeek score 70.20

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 7
pi: 0.012
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	GGCTGGCCTTTACGTAAAGA	50.0	50.0	58.1	57.9	713-715 bp	1.0	83.5
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGGCTGGCCTTTACGTAAAG	50.0	50.0	58.1	57.9	714-716 bp	1.0	83.4
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	726-728 bp	1.0	83.2
trnF-GAA-ndhJ_p4	TACGGGAAATGGTCGGGATA	GGCTGGCCTTTACGTAAAGA	50.0	50.0	57.9	57.9	763-765 bp	1.0	82.9
trnF-GAA-ndhJ_p5	TACGGGAAATGGTCGGGATA	AGGCTGGCCTTTACGTAAAG	50.0	50.0	57.9	57.9	764-766 bp	1.0	82.8