Hotspot

psaA-ycf3

LSC · 69605-70283 · 679 bp

Back to Crepidium

Pi 0.00739

MarkerSeek score 63.30

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 679
variable_sites: 5
pi: 0.00739
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	CCCATTCCTCGAAAGACGTT	ATAGCGCTTACTCCAGGGAA	50.0	50.0	57.9	58.2	825-827 bp	1.0	78.2
psaA-ycf3_p2	CCCATTCCTCGAAAGACGTT	TAGCGCTTACTCCAGGGAAT	50.0	50.0	57.9	58.2	824-826 bp	1.0	78.2
psaA-ycf3_p3	CCCATTCCTCGAAAGACGTT	GTTCGATCAAGCTGCTGAGT	50.0	50.0	57.9	58.3	859-861 bp	1.0	77.6
psaA-ycf3_p4	CCCATTCCTCGAAAGACGTT	GCTATAGCGCTTACTCCAGG	50.0	55.0	57.9	57.6	828-830 bp	1.0	77.1
psaA-ycf3_p5	CCCATTCCTCGAAAGACGTT	CTTGGTTCGATCAAGCTGCT	50.0	50.0	57.9	58.6	863-865 bp	1.0	75.5