Hotspot

rpoB-trnC-GCA

LSC · 53634-55033 · 1400 bp

Back to Crepidium

Pi 0.0101

MarkerSeek score 65.10

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1400
variable_sites: 14
pi: 0.0101
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AATTCTTCCGTCAAGCCCTG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1548-1556 bp	1.0	75.5
rpoB-trnC-GCA_p2	AATTCTTCCGTCAAGCCCTG	CCTTACCACTTGGCCATGTC	50.0	55.0	58.2	58.5	1527-1535 bp	1.0	75.5
rpoB-trnC-GCA_p3	AATTCTTCCGTCAAGCCCTG	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	1572-1580 bp	1.0	74.5
rpoB-trnC-GCA_p4	AATTCTTCCGTCAAGCCCTG	TTTGTTGATCAGGCGACACC	50.0	50.0	58.2	58.8	1585-1593 bp	1.0	73.6
rpoB-trnC-GCA_p5	AATTCTTCCGTCAAGCCCTG	CTTACCACTTGGCCATGTCG	50.0	55.0	58.2	58.9	1526-1534 bp	1.0	72.5