Hotspot

ycf1

SSC · 123126-128141 · 5016 bp

Back to Crepidiastrum

Pi 0.0315

MarkerSeek score 56.20

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5016
variable_sites: 157
pi: 0.0315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAATTCTCCTCTTTATTTGGTAAT	CATCGTCTAGATTAATCCCCC	25.0	47.6	52.4	55.1	169-172 bp	1.0	46.6
ycf1_p2	AAATTCTCCTCTTTATTTGGTAAT	ACATCGTCTAGATTAATCCCCC	25.0	45.5	52.4	56.8	170-173 bp	1.0	46.5
ycf1_p3	TTTAAATTCTCCTCTTTATTTGGT	CATCGTCTAGATTAATCCCCC	25.0	47.6	52.6	55.1	172-175 bp	1.0	46.5
ycf1_p4	TTTAAATTCTCCTCTTTATTTGGT	ACATCGTCTAGATTAATCCCCC	25.0	45.5	52.6	56.8	173-176 bp	1.0	46.4
ycf1_p5	TTTAAATTCTCCTCTTTATTTGGT	TACATCGTCTAGATTAATCCCCC	25.0	43.5	52.6	56.7	174-177 bp	1.0	46.4