Hotspot

ndhD-psaC

SSC · 116484-116597 · 114 bp

Back to Crepidiastrum

Pi 0.0877

MarkerSeek score 73.40

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 114
variable_sites: 10
pi: 0.0877
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTA	35.0	50.0	53.9	57.5	158-159 bp	1.0	48.6
ndhD-psaC_p2	TGTTAACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTA	33.3	50.0	57.8	57.5	162-163 bp	1.0	47.6
ndhD-psaC_p3	AACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTAT	35.0	47.6	53.9	58.0	158-159 bp	1.0	45.0
ndhD-psaC_p4	TGTTAACCAAGGAAATTTGTTCGT	CGCAGCATGGGTATAGCTTAT	33.3	47.6	57.8	58.0	162-163 bp	1.0	44.9
ndhD-psaC_p5	TGTTAACCAAGGAAATTTGTTCG	CGCAGCATGGGTATAGCTTA	34.8	50.0	56.2	57.5	162-163 bp	1.0	44.9