Hotspot

rpl32-trnL-UAG

SSC · 112616-113540 · 925 bp

Back to Crepidiastrum

Pi 0.0339

MarkerSeek score 73.10

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 925
variable_sites: 29
pi: 0.0339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGAAATCTCTTTCTACCGGG	AGCGTGTCTACCGATTTCAC	52.4	50.0	57.8	58.0	975-1017 bp	1.0	76.7
rpl32-trnL-UAG_p2	GGGAAATCTCTTTCTACCGGG	CAGCGTGTCTACCGATTTCA	52.4	50.0	57.8	58.0	976-1018 bp	1.0	76.7
rpl32-trnL-UAG_p3	GGGAAATCTCTTTCTACCGGG	TCCTAAGAGCAGCGTGTCTA	52.4	50.0	57.8	57.9	985-1027 bp	1.0	75.7
rpl32-trnL-UAG_p4	GGGAAATCTCTTTCTACCGGG	CCTAAGAGCAGCGTGTCTAC	52.4	55.0	57.8	57.8	984-1026 bp	1.0	75.2
rpl32-trnL-UAG_p5	GGGAAATCTCTTTCTACCGGG	CTAAGAGCAGCGTGTCTACC	52.4	55.0	57.8	57.8	983-1025 bp	1.0	75.2