Hotspot

ndhF-rpl32

SSC · 111338-112450 · 1113 bp

Back to Crepidiastrum

Pi 0.0214

MarkerSeek score 65.60

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1113
variable_sites: 23
pi: 0.0214
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGAAGGAAAGGT	CCGCCCAATATCCTTTCCTT	50.0	50.0	57.0	57.6	1227-1247 bp	1.0	73.4
ndhF-rpl32_p2	AAAGAAGAAGTCCCAACCCT	CCGCCCAATATCCTTTCCTT	45.0	50.0	56.2	57.6	1260-1280 bp	1.0	67.0
ndhF-rpl32_p3	CTGGAAGTGGAAGGAAAGGT	CCGCCCAATATCCTTTCCTTT	50.0	47.6	57.0	58.3	1227-1247 bp	1.0	65.7
ndhF-rpl32_p4	AAAAGAAGAAGTCCCAACCCT	CCGCCCAATATCCTTTCCTT	42.9	50.0	57.0	57.6	1261-1281 bp	1.0	65.2
ndhF-rpl32_p5	CTGGAAGTGGAAGGAAAGGTA	CCGCCCAATATCCTTTCCTT	47.6	50.0	56.9	57.6	1227-1247 bp	1.0	64.5