Hotspot

rpl16-rps3

LSC · 82443-82591 · 149 bp

Back to Crepidiastrum

Pi 0.042

MarkerSeek score 65.60

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 149
variable_sites: 6
pi: 0.042
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	AAATAAAGTTTTCGCGGGCG	AGTAATGGGGGAACATGGGA	45.0	50.0	58.0	58.0	1489-1497 bp	1.0	81.6
rpl16-rps3_p2	AAATAAAGTTTTCGCGGGCG	GTCGAGGGAATTGCACGTAT	45.0	50.0	58.0	58.1	1307-1315 bp	1.0	81.3
rpl16-rps3_p3	GGTTCCTTCGTTCCCATCTC	AGTAATGGGGGAACATGGGA	55.0	50.0	58.0	58.0	1333 bp	0.5	59.8
rpl16-rps3_p4	GGTTCCTTCGTTCCCATCTC	GTCGAGGGAATTGCACGTAT	55.0	50.0	58.0	58.1	1151 bp	0.5	59.3
rpl16-rps3_p5	GGTTCCTTCGTTCCCATCTC	GTAATGGGGGAACATGGGAC	55.0	55.0	58.0	57.9	1332 bp	0.5	59.3