Hotspot

rpl32-trnL

SSC · 113415-114289 · 875 bp

Back to Cremolobus

Pi 0.0344

MarkerSeek score 66.90

Primer available yes

Label rpl32-trnL

Feature Metadata

label_name: rpl32-trnL
feature_type: igs
region: SSC
length_bp: 875
variable_sites: 30
pi: 0.0344
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL_p1	AACGAGCACTCTAGTACGGA	TGCAGACTGAGGCATTTTGT	50.0	45.0	57.9	58.0	1835-1879 bp	1.0	80.9
rpl32-trnL_p2	AACGAGCACTCTAGTACGGA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1305-1341 bp	1.0	80.7
rpl32-trnL_p3	AACGAGCACTCTAGTACGGA	TAACCACTGGGACTGGAGAG	50.0	55.0	57.9	58.1	1868-1912 bp	1.0	80.5
rpl32-trnL_p4	AACGAGCACTCTAGTACGGA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	1298-1334 bp	1.0	80.2
rpl32-trnL_p5	AACGAGCACTCTAGTACGGA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	1303-1339 bp	1.0	80.1