Hotspot

rpl36-rps8

LSC · 79187-79669 · 483 bp

Back to Cremolobus

Pi 0.0231

MarkerSeek score 67.50

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 483
variable_sites: 11
pi: 0.0231
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	GTTTATGCCTCGGGTTGGAA	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	609-615 bp	1.0	77.7
rpl36-rps8_p2	TTTATGCCTCGGGTTGGAAC	TCGGCGGAGAAATTTTGTGT	50.0	45.0	58.2	58.4	608-614 bp	1.0	77.7
rpl36-rps8_p3	GTTTATGCCTCGGGTTGGAA	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	608-614 bp	1.0	77.5
rpl36-rps8_p4	TTTATGCCTCGGGTTGGAAC	CGGCGGAGAAATTTTGTGTT	50.0	45.0	58.2	57.6	607-613 bp	1.0	77.5
rpl36-rps8_p5	TTTATGCCTCGGGTTGGAAC	TCCACGAATTTTAGGCGGAA	50.0	45.0	58.2	57.5	694-700 bp	1.0	76.8