Hotspot

trnT-trnL

LSC · 46290-47104 · 815 bp

Back to Cremolobus

Pi 0.0233

MarkerSeek score 66.30

Primer available yes

Label trnT-trnL

Feature Metadata

label_name: trnT-trnL
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 19
pi: 0.0233
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-trnL_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCAATTCC	55.0	55.0	57.9	58.2	876-891 bp	1.0	79.5
trnT-trnL_p2	TATGGAAAAGCCGGCTATCG	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	927-942 bp	1.0	79.2
trnT-trnL_p3	GCGATGCTCTAACCTCTGAG	AGTCCGTAGCGTCTACCAAT	55.0	50.0	57.9	58.2	879-894 bp	1.0	78.8
trnT-trnL_p4	GGAATCGAACCGATGACCAT	CCGTAGCGTCTACCAATTCC	50.0	55.0	57.8	58.2	908-923 bp	1.0	78.6
trnT-trnL_p5	TATGGAAAAGCCGGCTATCG	AGTCCGTAGCGTCTACCAAT	50.0	50.0	57.8	58.2	930-945 bp	1.0	78.5