Hotspot

psbC-trnS

LSC · 35204-35384 · 181 bp

Back to Cremolobus

Pi 0.0444

MarkerSeek score 71.10

Primer available yes

Label psbC-trnS

Feature Metadata

label_name: psbC-trnS
feature_type: igs
region: LSC
length_bp: 181
variable_sites: 8
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbC-trnS_p1	TCGTGGGTCATTTATGGCAC	GATGGCTCCGGTCTTGAAAA	50.0	50.0	58.3	58.2	363-364 bp	1.0	78.5
psbC-trnS_p2	TCGTGGGTCATTTATGGCAC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	58.3	58.2	313-314 bp	1.0	78.5
psbC-trnS_p3	CGGCAGCAGGATTTGAAAAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	57.6	58.2	274-275 bp	1.0	77.4
psbC-trnS_p4	CGGCAGCAGGATTTGAAAAA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.6	58.2	324-325 bp	1.0	77.3
psbC-trnS_p5	TTCGTGGGTCATTTATGGCA	GATGGCTCCGGTCTTGAAAA	45.0	50.0	57.5	58.2	364-365 bp	1.0	76.6