Hotspot

rpoB-trnC

LSC · 26388-27482 · 1095 bp

Back to Cremolobus

Pi 0.0239

MarkerSeek score 66.20

Primer available yes

Label rpoB-trnC

Feature Metadata

label_name: rpoB-trnC
feature_type: igs
region: LSC
length_bp: 1095
variable_sites: 26
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC_p1	GTTCCCTCTTTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1183-1200 bp	1.0	80.5
rpoB-trnC_p2	GTTCCCTCTTTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1165-1182 bp	1.0	78.4
rpoB-trnC_p3	GTTCCCTCTTTTCCATCCCC	TCAAGTCTGTTGTTGAGGCG	55.0	50.0	57.9	58.4	1207-1224 bp	1.0	77.8
rpoB-trnC_p4	TGTTCCCTCTTTTCCATCCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.1	58.1	1184-1201 bp	1.0	74.5
rpoB-trnC_p5	GTTCCCTCTTTTCCATCCCC	AAGTCTGTTGTTGAGGCGG	55.0	52.6	57.9	58.0	1205-1222 bp	1.0	73.1