Hotspot

atpH-atpI

LSC · 13576-14068 · 493 bp

Back to Cremolobus

Pi 0.0329

MarkerSeek score 68.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 493
variable_sites: 16
pi: 0.0329
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACCGAAGCAGCAGAAATCA	TACAAGCGGGATTCAAGCTC	45.0	50.0	58.1	58.0	599-602 bp	1.0	81.9
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	657-660 bp	1.0	81.6
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	TACAAGCGGGATTCAAGCTC	50.0	50.0	57.9	58.0	639-642 bp	1.0	81.5
atpH-atpI_p4	AACCGAAGCAGCAGAAATCA	TTTTTGCCACTTTAGCTGCG	45.0	45.0	58.1	57.9	576-579 bp	1.0	81.2
atpH-atpI_p5	GCTGTACCTTGACCAACTCC	TACAAGCGGGATTCAAGCTC	55.0	50.0	58.2	58.0	661-664 bp	1.0	80.9