Hotspot

atpF-atpH

LSC · 12868-13329 · 462 bp

Back to Cremolobus

Pi 0.0238

MarkerSeek score 69.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 462
variable_sites: 11
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	580-596 bp	1.0	77.2
atpF-atpH_p2	CCAGTGACCCAAGTAAACGA	TGGCCTGGTTGTAGCATTAG	50.0	50.0	57.5	57.6	553-569 bp	1.0	73.9
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TTTATGGCCTGGTTGTAGCA	50.0	45.0	57.9	57.1	584-600 bp	1.0	73.4
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GGCCTGGTTGTAGCATTAGC	50.0	55.0	57.9	59.0	579-595 bp	1.0	72.8
atpF-atpH_p5	GGCCAGTGACCCAAGTAAAC	TGGCCTGGTTGTAGCATTAG	55.0	50.0	58.8	57.6	555-571 bp	1.0	72.2