Hotspot

trnS-trnG

LSC · 8007-8654 · 648 bp

Back to Cremolobus

Pi 0.0237

MarkerSeek score 61.40

Primer available yes

Label trnS-trnG

Feature Metadata

label_name: trnS-trnG
feature_type: igs
region: LSC
length_bp: 648
variable_sites: 15
pi: 0.0237
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-trnG_p1	AGTCCACTCAGCCATCTCTC	AATCAAACCGAGAGACCCTT	55.0	45.0	58.5	56.5	743-746 bp	1.0	66.8
trnS-trnG_p2	AGTCCACTCAGCCATCTCTC	ATCAAACCGAGAGACCCTTT	55.0	45.0	58.5	56.5	742-745 bp	1.0	66.8
trnS-trnG_p3	AGTCCACTCAGCCATCTCTC	AATCAAACCGAGAGACCCTTT	55.0	42.9	58.5	57.2	743-746 bp	1.0	64.9
trnS-trnG_p4	TAGTCCACTCAGCCATCTCT	AATCAAACCGAGAGACCCTT	50.0	45.0	57.2	56.5	744-747 bp	1.0	64.2
trnS-trnG_p5	TAGTCCACTCAGCCATCTCT	ATCAAACCGAGAGACCCTTT	50.0	45.0	57.2	56.5	743-746 bp	1.0	64.2