Hotspot

psbI-trnS

LSC · 7828-7918 · 91 bp

Back to Cremolobus

Pi 0.044

MarkerSeek score 67.60

Primer available yes

Label psbI-trnS

Feature Metadata

label_name: psbI-trnS
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 4
pi: 0.044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS_p1	AATGATCCCGGACGTAATCC	TTAATCGTACCGAGGGTTCG	50.0	50.0	57.2	57.2	163 bp	1.0	68.5
psbI-trnS_p2	AATGATCCCGGACGTAATCC	GGGTTCGAATCCCTCTCTTT	50.0	50.0	57.2	56.9	150 bp	1.0	66.8
psbI-trnS_p3	AATGATCCCGGACGTAATCC	GTTCGAATCCCTCTCTTTCCC	50.0	52.4	57.2	58.1	148 bp	1.0	66.8
psbI-trnS_p4	AATGATCCCGGACGTAATCC	GGGTTCGAATCCCTCTCTTTC	50.0	52.4	57.2	58.1	150 bp	1.0	66.7
psbI-trnS_p5	AATGATCCCGGACGTAATCC	GGTTCGAATCCCTCTCTTTCC	50.0	52.4	57.2	58.1	149 bp	1.0	66.7