Hotspot

trnH-psbA

LSC · 77-397 · 321 bp

Back to Cremolobus

Pi 0.0381

MarkerSeek score 72.40

Primer available yes

Label trnH-psbA

Feature Metadata

label_name: trnH-psbA
feature_type: igs
region: LSC
length_bp: 321
variable_sites: 12
pi: 0.0381
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-psbA_p1	CTGCCTTAATCCACTTGGCT	GCTGTTGAGGCTCCATCTAC	50.0	55.0	57.9	58.1	378-379 bp	1.0	82.3
trnH-psbA_p2	CTGCCTTAATCCACTTGGCT	CCTCTAGACCTAGCTGCTGT	50.0	55.0	57.9	57.9	393-394 bp	1.0	82.1
trnH-psbA_p3	ACTGCCTTAATCCACTTGGC	GCTGTTGAGGCTCCATCTAC	50.0	55.0	58.2	58.1	379-380 bp	1.0	82.1
trnH-psbA_p4	CTGCCTTAATCCACTTGGCT	TTCCCTCTAGACCTAGCTGC	50.0	55.0	57.9	57.9	396-397 bp	1.0	82.0
trnH-psbA_p5	ACTGCCTTAATCCACTTGGC	CCTCTAGACCTAGCTGCTGT	50.0	55.0	58.2	57.9	394-395 bp	1.0	81.8