Pi
0.00399
MarkerSeek score
55.60
Primer available
yes
Label
ycf1
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| ycf1_p1 | CCCCGAACTTCTTCGTAGTG | GCGGTGGTCGTATTTCTTCT | 55.0 | 50.0 | 58.0 | 58.0 | 921-942 bp | 1.0 | 82.1 |
| ycf1_p2 | CCCGAACTTCTTCGTAGTGG | GCGGTGGTCGTATTTCTTCT | 55.0 | 50.0 | 58.0 | 58.0 | 920-941 bp | 1.0 | 82.1 |
| ycf1_p3 | GAGCTACTGAGGAACAACGG | GCGGTGGTCGTATTTCTTCT | 55.0 | 50.0 | 58.0 | 58.0 | 1016-1037 bp | 1.0 | 82.0 |
| ycf1_p4 | GGCAGAATAGCAGAGCAAGT | GCGGTGGTCGTATTTCTTCT | 50.0 | 50.0 | 58.0 | 58.0 | 1313-1334 bp | 1.0 | 81.2 |
| ycf1_p5 | GTCCCCCATCAGCGTATTTT | GCGGTGGTCGTATTTCTTCT | 50.0 | 50.0 | 57.9 | 58.0 | 1373-1394 bp | 1.0 | 81.0 |
| ycf1_p1 | TGTATCTTAATGGACGATTCC | ACTGAACAAACTCGACAAAATGG | 38.1 | 39.1 | 52.9 | 58.1 | 197-200 bp | 1.0 | 44.5 |
| ycf1_p2 | TGTATCTTAATGGACGATTCC | CTGAACAAACTCGACAAAATGGA | 38.1 | 39.1 | 52.9 | 57.8 | 196-199 bp | 1.0 | 44.5 |
| ycf1_p3 | TGTATCTTAATGGACGATTCC | TGAACAAACTCGACAAAATGGA | 38.1 | 36.4 | 52.9 | 56.8 | 195-198 bp | 1.0 | 44.5 |
| ycf1_p4 | TGTATCTTAATGGACGATTCC | ACCGTCACCCATTTTTAGTAAGA | 38.1 | 39.1 | 52.9 | 57.8 | 221-224 bp | 1.0 | 44.0 |
| ycf1_p5 | TGTATCTTAATGGACGATTCC | CGACAAAATGGAAGAAGAAGAGG | 38.1 | 43.5 | 52.9 | 57.7 | 185 bp | 0.8 | 33.3 |