Hotspot

ndhC-trnV-UAC

LSC · 50346-51114 · 769 bp

Back to Cremanthodium

Pi 0.00431

MarkerSeek score 63.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 769
variable_sites: 7
pi: 0.00431
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCTTTGCTAATCGGAGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.1	57.8	906-913 bp	1.0	72.7
ndhC-trnV-UAC_p2	TCGGAGCTAAAAATCCCGAA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	56.9	57.8	895-902 bp	1.0	71.5
ndhC-trnV-UAC_p3	CCCTTTGCTAATCGGAGCTAA	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.8	57.8	906-913 bp	1.0	70.5
ndhC-trnV-UAC_p4	CCCTTTGCTAATCGGAGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	57.1	57.5	905-912 bp	1.0	70.0
ndhC-trnV-UAC_p5	GGCCCTTTGCTAATCGGAG	AGAAGGTCTACGGTTCGAGT	57.9	50.0	58.3	57.8	908-915 bp	1.0	69.7