Hotspot

ycf3-trnS-GGA

LSC · 44032-44923 · 892 bp

Back to Cremanthodium

Pi 0.0028

MarkerSeek score 57.00

Primer available yes

Label ycf3-trnS-GGA

Feature Metadata

label_name: ycf3-trnS-GGA
feature_type: igs
region: LSC
length_bp: 892
variable_sites: 6
pi: 0.0028
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS-GGA_p1	TTCTCCTGAAGTTGTCGGAA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1019-1023 bp	1.0	66.6
ycf3-trnS-GGA_p2	TTTCTCCTGAAGTTGTCGGA	ATGCTACGCTTTCAACCACT	45.0	45.0	56.4	57.8	1020-1024 bp	1.0	66.6
ycf3-trnS-GGA_p3	TTCTCCTGAAGTTGTCGGAA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1062-1066 bp	1.0	66.2
ycf3-trnS-GGA_p4	TTTCTCCTGAAGTTGTCGGA	GGATTCGAACCCTCGGTAAG	45.0	55.0	56.4	57.8	1063-1067 bp	1.0	66.2
ycf3-trnS-GGA_p5	TTCTCCTGAAGTTGTCGGAA	CCAATGCTACGCTTTCAACC	45.0	50.0	56.4	57.7	1022-1026 bp	1.0	65.9