Hotspot

atpH-atpF

LSC · 26471-26866 · 396 bp

Back to Cremanthodium

Pi 0.0081

MarkerSeek score 54.20

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: LSC
length_bp: 396
variable_sites: 8
pi: 0.0081
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CGAGGCGGAGGGAAAAATAA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.0	57.9	548-552 bp	1.0	81.0
atpH-atpF_p2	CGAGGCGGAGGGAAAAATAA	ATTAAACCCGAAACTCCCGG	50.0	50.0	58.0	57.9	579-583 bp	1.0	80.4
atpH-atpF_p3	CGAGGCGGAGGGAAAAATAA	GTGGCCCAAAGAAACGAAAG	50.0	50.0	58.0	57.9	549-553 bp	1.0	80.4
atpH-atpF_p4	CGAGGCGGAGGGAAAAATAA	AGTGGCCCAAAGAAACGAAA	50.0	45.0	58.0	58.2	550-554 bp	1.0	79.6
atpH-atpF_p5	GCCTGGTTGTAGCATTAGCA	TGGCCCAAAGAAACGAAAGA	50.0	45.0	58.2	57.9	477-481 bp	1.0	79.5