Hotspot

petA

LSC · 60200-61159 · 960 bp

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Pi 0.00104

MarkerSeek score 49.60

Primer available yes

Label petA

Feature Metadata

label_name: petA
feature_type: gene
region: LSC
length_bp: 960
variable_sites: 1
pi: 0.00104
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA_p1	ACTAGCGACTTACCCTCCTT	TGGTTCTTTTTACGAACTTGGTG	50.0	39.1	57.5	57.8	1058 bp	1.0	49.9
petA_p2	ACTAGCGACTTACCCTCCTT	GGTTCTTTTTACGAACTTGGTGT	50.0	39.1	57.5	57.8	1057 bp	1.0	49.9
petA_p3	ACTAGCGACTTACCCTCCTT	TGGTTCTTTTTACGAACTTGGT	50.0	36.4	57.5	56.4	1058 bp	1.0	47.1
petA_p4	ACTAGCGACTTACCCTCCTT	GGTTCTTTTTACGAACTTGGTG	50.0	40.9	57.5	56.1	1057 bp	1.0	44.9
petA_p5	ACTAGCGACTTACCCTCCTT	TTGGTTCTTTTTACGAACTTGGT	50.0	34.8	57.5	57.1	1059 bp	1.0	44.6