Hotspot

rpl22-rps19

LSC · 80986-81043 · 58 bp

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Pi 0.0172

MarkerSeek score 62.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 58
variable_sites: 1
pi: 0.0172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GGACCGTCCACGAATTTGAT	AGGAGCACCTGCCTATTTTT	50.0	45.0	58.3	57.1	291 bp	1.0	70.9
rpl22-rps19_p2	TAGGACCGTCCACGAATTTG	AGGAGCACCTGCCTATTTTT	50.0	45.0	57.6	57.1	293 bp	1.0	70.0
rpl22-rps19_p3	ACCGTCCACGAATTTGATCA	AGGAGCACCTGCCTATTTTT	45.0	45.0	57.5	57.1	289 bp	1.0	69.3
rpl22-rps19_p4	GGACCGTCCACGAATTTGAT	TGGTAGGCCACAAATTAGGAG	50.0	47.6	58.3	57.4	258 bp	1.0	65.0
rpl22-rps19_p5	GACCGTCCACGAATTTGATC	AGGAGCACCTGCCTATTTTT	50.0	45.0	56.9	57.1	290 bp	1.0	64.3