Hotspot

rpl14-rpl16

LSC · 78422-78560 · 139 bp

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Pi 0.0152

MarkerSeek score 66.00

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 139
variable_sites: 2
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGCGCCTACGATTCGAATAC	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.0	57.9	262-269 bp	1.0	81.1
rpl14-rpl16_p2	AGCGCCTACGATTCGAATAC	TAGCAGCATCCAAAATGCCT	50.0	45.0	58.0	57.9	263-270 bp	1.0	81.1
rpl14-rpl16_p3	AGCGCCTACGATTCGAATAC	CGGGCAATTTCAATAGCAGC	50.0	50.0	58.0	58.2	276-283 bp	1.0	80.9
rpl14-rpl16_p4	CCGCTGTTATCGGCTACATT	AGCAGCATCCAAAATGCCTA	50.0	45.0	58.1	57.9	225-232 bp	1.0	80.9
rpl14-rpl16_p5	CCGCTGTTATCGGCTACATT	TAGCAGCATCCAAAATGCCT	50.0	45.0	58.1	57.9	226-233 bp	1.0	80.9