Hotspot

accD-psaI

LSC · 57061-57713 · 653 bp

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Pi 0

MarkerSeek score 27.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 653
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 27.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTCCACGCTTTCTTTCCCTT	CACAAAAATAGAGGGAAAACTGA	45.0	34.8	57.6	55.1	734-735 bp	1.0	40.0
accD-psaI_p2	AGTTCCACGCTTTCTTTCCC	CACAAAAATAGAGGGAAAACTGA	50.0	34.8	58.4	55.1	736-737 bp	1.0	40.0
accD-psaI_p3	GTTCCACGCTTTCTTTCCCT	CACAAAAATAGAGGGAAAACTGA	50.0	34.8	58.4	55.1	735-736 bp	1.0	40.0
accD-psaI_p4	CCACGCTTTCTTTCCCTTGA	CACAAAAATAGAGGGAAAACTGA	50.0	34.8	58.4	55.1	732-733 bp	1.0	40.0
accD-psaI_p5	TCCACGCTTTCTTTCCCTTG	CACAAAAATAGAGGGAAAACTGA	50.0	34.8	58.4	55.1	733-734 bp	1.0	40.0