Hotspot

trnF-GAA-ndhJ

LSC · 47181-47784 · 604 bp

Back to Crawfurdia

Pi 0.00678

MarkerSeek score 64.90

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 4
pi: 0.00678
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	738-752 bp	1.0	81.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCTGGTAGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	737-751 bp	1.0	81.6
trnF-GAA-ndhJ_p3	GGATGCTACATTGGGACTGG	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	760-774 bp	1.0	81.4
trnF-GAA-ndhJ_p4	GGATGCTACATTGGGACTGG	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	759-773 bp	1.0	81.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	701-715 bp	1.0	81.3