Hotspot

trnT-GGU-psbD

LSC · 30554-31617 · 1064 bp

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Pi 0.00564
MarkerSeek score 62.00
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1064
variable_sites
6
pi
0.00564
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG GAAACGGTCCCTACGTAACC 55.0 55.0 58.1 58.0 1206-1232 bp 1.0 79.9
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA GAAACGGTCCCTACGTAACC 50.0 55.0 58.1 58.0 1209-1235 bp 1.0 79.7
trnT-GGU-psbD_p3 GTGGTAGAGTAACGCCATGG GACCAGCCTACAAACACGAA 55.0 50.0 58.1 58.1 1223-1249 bp 1.0 78.9
trnT-GGU-psbD_p4 GTGGTAGAGTAACGCCATGG AAACACGAAACGGTCCCTAC 55.0 50.0 58.1 58.1 1212-1238 bp 1.0 78.8
trnT-GGU-psbD_p5 GTGGTAGAGTAACGCCATGG CAAACACGAAACGGTCCCTA 55.0 50.0 58.1 58.1 1213-1239 bp 1.0 78.8