Hotspot

rpoB-trnC-GCA

LSC · 25222-26444 · 1223 bp

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Pi 0.00491

MarkerSeek score 61.70

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1223
variable_sites: 6
pi: 0.00491
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCTTCCGTCAAACCCTCA	GCGATTTTTATCAGCCGACG	50.0	50.0	57.8	58.0	1441 bp	1.0	78.3
rpoB-trnC-GCA_p2	GTTCTTCCGTCAAACCCTCA	TTTTATCAGCCGACGAAGGG	50.0	50.0	57.8	58.0	1436 bp	1.0	78.2
rpoB-trnC-GCA_p3	GTTCTTCCGTCAAACCCTCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.8	58.1	1388 bp	1.0	77.7
rpoB-trnC-GCA_p4	GTTCTTCCGTCAAACCCTCA	GACGAAGGGATTTCAAGGCT	50.0	50.0	57.8	57.9	1425 bp	1.0	77.5
rpoB-trnC-GCA_p5	CTTCCGTCAAACCCTCATCA	GCGATTTTTATCAGCCGACG	50.0	50.0	57.5	58.0	1438 bp	1.0	76.6