Hotspot

trnK-UUU-trnQ-UUG

LSC · 4218-5484 · 1267 bp

Back to Crawfurdia

Pi 0.00592
MarkerSeek score 66.50
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1267
variable_sites
7
pi
0.00592
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 CGAATCCCTTGCTTCATCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1373-1814 bp 1.0 83.4
trnK-UUU-trnQ-UUG_p2 ACGAATCCCTTGCTTCATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1374-1815 bp 1.0 83.4
trnK-UUU-trnQ-UUG_p3 ATGGACGAATCCCTTGCTTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1378-1819 bp 1.0 83.4
trnK-UUU-trnQ-UUG_p4 GGACGAATCCCTTGCTTCAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1376-1817 bp 1.0 83.4
trnK-UUU-trnQ-UUG_p5 CGATGGTATGGACGAATCCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.9 58.1 1385-1826 bp 1.0 83.1