Hotspot

trnH-GUG-psbA

LSC · 74-379 · 306 bp

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Pi 0.0131

MarkerSeek score 60.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 306
variable_sites: 4
pi: 0.0131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	430-431 bp	1.0	77.2
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	431-432 bp	1.0	77.0
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	431-432 bp	1.0	76.7
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.2	57.6	432-433 bp	1.0	76.5
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.7	58.3	423-424 bp	1.0	75.6