Hotspot

rpl16

LSC · 83440-85054 · 1615 bp

Back to Cratoxylum

Pi 0.0138

MarkerSeek score 54.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1615
variable_sites: 33
pi: 0.0138
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTCCTTGATCACCGCAACAA	CCCTACAAACCGTTCAAGCT	45.0	50.0	58.0	58.1	2135-2159 bp	1.0	81.7
rpl16_p2	CCCAGCAATTGCACCAAAAA	CCCTACAAACCGTTCAAGCT	45.0	50.0	58.0	58.1	2312-2336 bp	1.0	81.4
rpl16_p3	CCGCTGTTATCGGCTACATT	CCCTACAAACCGTTCAAGCT	50.0	50.0	58.1	58.1	2044-2068 bp	1.0	80.9
rpl16_p4	GATCTCCGCTGCCATCTTAG	CCCTACAAACCGTTCAAGCT	55.0	50.0	57.9	58.1	2405-2426 bp	0.666667	67.5
rpl16_p5	CCTACTTCCTTGATCACCGC	CCCTACAAACCGTTCAAGCT	55.0	50.0	58.1	58.1	2164 bp	0.333333	52.0