Hotspot

clpP

LSC · 71894-73497 · 1604 bp

Back to Cratoxylum

Pi 0.0186

MarkerSeek score 61.10

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1604
variable_sites: 44
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	ATTCAGCCGGACATAAGCAG	ACTTGGTTCATTACACGCTT	50.0	40.0	58.0	55.6	1718-1754 bp	1.0	62.9
clpP_p2	ATTCAGCCGGACATAAGCAG	ACTTGGTTCATTACACGCTTT	50.0	38.1	58.0	56.4	1718-1754 bp	1.0	61.2
clpP_p3	AATTCAGCCGGACATAAGCA	ACTTGGTTCATTACACGCTT	45.0	40.0	57.6	55.6	1719-1755 bp	1.0	59.9
clpP_p4	ATTCAGCCGGACATAAGCAG	ACTTACTTGGTTCATTACACGC	50.0	40.9	58.0	57.0	1722-1758 bp	1.0	58.2
clpP_p5	AATTCAGCCGGACATAAGCA	ACTTGGTTCATTACACGCTTT	45.0	38.1	57.6	56.4	1719-1755 bp	1.0	58.2