Hotspot

rpl32-trnL-UAG

SSC · 115856-116556 · 701 bp

Back to Cratoxylum

Pi 0.0358
MarkerSeek score 68.80
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
701
variable_sites
37
pi
0.0358
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGATATTGGGCAGCCTTGAA AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 830-835 bp 1.0 84.6
rpl32-trnL-UAG_p2 GGATATTGGGCAGCCTTGAA CAGCGTGTCTACCGATTTCA 50.0 50.0 57.9 58.0 831-836 bp 1.0 84.5
rpl32-trnL-UAG_p3 AAGGATATTGGGCAGCCTTG AGCGTGTCTACCGATTTCAC 50.0 50.0 57.9 58.0 832-837 bp 1.0 84.5
rpl32-trnL-UAG_p4 TTGTTGCTTGCGGAAAAAGG AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 1212-1508 bp 1.0 82.8
rpl32-trnL-UAG_p5 TTGTTGCTTGCGGAAAAAGG CAGCGTGTCTACCGATTTCA 45.0 50.0 58.1 58.0 1213-1509 bp 1.0 82.8