Hotspot

petD-rpoA

LSC · 79642-79825 · 184 bp

Back to Cratoxylum

Pi 0.0383

MarkerSeek score 66.10

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 184
variable_sites: 10
pi: 0.0383
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	AGTCGCGACAACTGTCTTTT	ACAGGGCTACTTTACCCCTT	45.0	50.0	58.1	57.9	582-592 bp	1.0	82.6
petD-rpoA_p2	GTTTCAGTACCAGCGGGATT	ACAGGGCTACTTTACCCCTT	50.0	50.0	57.9	57.9	659-669 bp	1.0	82.1
petD-rpoA_p3	AGTCGCGACAACTGTCTTTT	ACGCCATATTCATGCCTGTT	45.0	45.0	58.1	57.9	795-805 bp	1.0	81.8
petD-rpoA_p4	GTTTCAGTACCAGCGGGATT	ACGCCATATTCATGCCTGTT	50.0	45.0	57.9	57.9	872-882 bp	1.0	81.4
petD-rpoA_p5	AGTCGCGACAACTGTCTTTT	AGACGCCATATTCATGCCTG	45.0	50.0	58.1	58.1	797-807 bp	1.0	81.4