Hotspot

petG-trnW-CCA

LSC · 67528-67640 · 113 bp

Back to Cratoxylum

Pi 0.0413

MarkerSeek score 70.90

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 113
variable_sites: 7
pi: 0.0413
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	ACAGACGTGGTGATCAGTTG	AGAACGTGGGTCTCCAAAAC	50.0	50.0	57.8	58.0	195 bp	1.0	81.3
petG-trnW-CCA_p2	ACAGACGTGGTGATCAGTTG	CAGAACGTGGGTCTCCAAAA	50.0	50.0	57.8	58.0	196 bp	1.0	81.3
petG-trnW-CCA_p3	ACAGACGTGGTGATCAGTTG	AGTTCAGTTTGGCAGAACGT	50.0	45.0	57.8	58.0	208 bp	1.0	81.1
petG-trnW-CCA_p4	ACAGACGTGGTGATCAGTTG	GTTCAGTTTGGCAGAACGTG	50.0	50.0	57.8	57.9	207 bp	1.0	80.6
petG-trnW-CCA_p5	CAGACGTGGTGATCAGTTGG	AGAACGTGGGTCTCCAAAAC	55.0	50.0	58.6	58.0	194 bp	1.0	78.1