Hotspot

ndhA

SSC · 121535-123723 · 2189 bp

Back to Crateva

Pi 0.00914

MarkerSeek score 62.60

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2189
variable_sites: 20
pi: 0.00914
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	CCCGGTTATCATAGGAAGCA	GCCCACCCGGTTTTATTAAT	50.0	45.0	56.8	56.1	2411-2418 bp	1.0	59.3
ndhA_p2	ACCCGGTTATCATAGGAAGC	GCCCACCCGGTTTTATTAAT	50.0	45.0	56.8	56.1	2412-2419 bp	1.0	59.3
ndhA_p3	CCCGGTTATCATAGGAAGCA	TGACGATACTCGGTAGCATAGA	50.0	45.5	56.8	58.0	2342-2349 bp	1.0	58.9
ndhA_p4	ACCCGGTTATCATAGGAAGC	TGACGATACTCGGTAGCATAGA	50.0	45.5	56.8	58.0	2343-2350 bp	1.0	58.9
ndhA_p5	CCCGGTTATCATAGGAAGCA	ATGACGATACTCGGTAGCAT	50.0	45.0	56.8	55.9	2343-2350 bp	1.0	58.5