Hotspot

ndhF-rpl32

SSC · 114138-115185 · 1048 bp

Back to Crateva

Pi 0.029

MarkerSeek score 62.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1048
variable_sites: 29
pi: 0.029
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTGTTGCTGTCGGAAAAAGT	TTTTAGAGATAGAAGTACGTTTTT	40.0	25.0	56.4	52.2	1163-1185 bp	1.0	46.0
ndhF-rpl32_p2	TTGTTGCTGTCGGAAAAAGT	TTTTTAGAGATAGAAGTACGTTTT	40.0	25.0	56.4	52.2	1164-1186 bp	1.0	46.0
ndhF-rpl32_p3	TTTGTTGCTGTCGGAAAAAGT	TTTTAGAGATAGAAGTACGTTTTT	38.1	25.0	57.1	52.2	1164-1186 bp	1.0	46.0
ndhF-rpl32_p4	TTTGTTGCTGTCGGAAAAAGT	TTTTTAGAGATAGAAGTACGTTTT	38.1	25.0	57.1	52.2	1165-1187 bp	1.0	46.0
ndhF-rpl32_p5	TGCTGTCGGAAAAAGTAGAAGT	TTTTAGAGATAGAAGTACGTTTTT	40.9	25.0	57.9	52.2	1159-1181 bp	1.0	46.0