Hotspot

psaJ-rpl33

LSC · 68280-68722 · 443 bp

Back to Crateva

Pi 0.0339

MarkerSeek score 77.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 443
variable_sites: 22
pi: 0.0339
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGTTCGGTTCTTTAGCAGG	TCCTTTCGAACACAACTGGT	50.0	45.0	57.8	57.3	591-594 bp	1.0	76.3
psaJ-rpl33_p2	GGTTCGGTTCTTTAGCAGGT	TCCTTTCGAACACAACTGGT	50.0	45.0	57.8	57.3	590-593 bp	1.0	76.3
psaJ-rpl33_p3	TGGTTCGGTTCTTTAGCAGG	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	547-550 bp	1.0	74.3
psaJ-rpl33_p4	GGTTCGGTTCTTTAGCAGGT	AACATCTTTACCCTTGGCCA	50.0	45.0	57.8	57.0	546-549 bp	1.0	74.3
psaJ-rpl33_p5	TGGTTCGGTTCTTTAGCAGG	GAACATCTTTACCCTTGGCCA	50.0	47.6	57.8	58.2	548-551 bp	1.0	72.6