Hotspot

trnP-CCA-psaJ

LSC · 67765-68144 · 380 bp

Back to Crateva

Pi 0.00439

MarkerSeek score 57.60

Primer available yes

Label trnP-CCA-psaJ

Feature Metadata

label_name: trnP-CCA-psaJ
feature_type: igs
region: LSC
length_bp: 380
variable_sites: 2
pi: 0.00439
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-CCA-psaJ_p1	ACCCGTGACATTTTGTACCC	CCTGCTAAAGAACCGAACCA	50.0	50.0	58.1	57.8	496-506 bp	1.0	79.7
trnP-CCA-psaJ_p2	CCCGTGACATTTTGTACCCA	CCTGCTAAAGAACCGAACCA	50.0	50.0	58.1	57.8	495-505 bp	1.0	79.7
trnP-CCA-psaJ_p3	ACCCGTGACATTTTGTACCC	ACCTGCTAAAGAACCGAACC	50.0	50.0	58.1	57.8	497-507 bp	1.0	79.7
trnP-CCA-psaJ_p4	CCCGTGACATTTTGTACCCA	ACCTGCTAAAGAACCGAACC	50.0	50.0	58.1	57.8	496-506 bp	1.0	79.7
trnP-CCA-psaJ_p5	ACAGGATTTGAACCCGTGAC	CCTGCTAAAGAACCGAACCA	50.0	50.0	58.1	57.8	507-517 bp	1.0	79.6